polski | english | login
Array|Array|Array|Array|Array|Array

DOI: 10.18544/PEDM-22.03.0061

Permanent neonatal diabetes mellitus in a young Ukrainian child

Viktoriya Furdela, Halyna Pavlyshyn, Hryhorii Korytskyi, Alla Filiuk

Key words 

Wolcott-Rallison Syndrome, neonatal diabetes

Abstract

Diabetes mellitus is a chronic metabolic disease with the manifestation possible in any period of life. The incidence of diabetes is rising around the world, and studies show that children are at an increasing risk of developing the disease. Type 1 diabetes accounts for over 90% of childhood and adolescent diabetes, although less than 10% of children suffer from type 2 diabetes. Over the last few decades, inherited monogenic forms of DM were discovered and studied. An extremely rare form of diabetes (less than 1-2% of all diabetes in young people), with neonatal diabetes as a subset, and is usually suspected if a child is diagnosed with diabetes at less than 6 months of age. We present the first case reported in Ukraine of a child diagnosed with permanent neonatal diabetes resulting from a EIF2AK3 gene missense mutation of exon 15 (WolcottRallison Syndrome). Despite low incidence of the permanent neonatal diabetes, this diagnosis should be considered in infants with persistent hyperglycaemia requiring insulin therapy. 


Article published in polish language

Introduction

Diabetes mellitus is a chronic metabolic disease with manifestations possible in any period of life. The incidence of diabetes is rising around the world, and studies show that children are at an increasing risk of developing the disease. Type 1 diabetes accounts for over 90% of childhood and adolescent diabetes, although less than 10% of children suffer from type 2 diabetes. Over the last few decades, inherited monogenic forms of DM were discovered and studied. It is anextremely rare form of diabetes (less than 1–2% of all diabetes in young people), with neonatal diabetes as a subset, and is usually suspected if a child is diagnosed with diabetes at less than 6 months of age. About one in half a million children all over the world are diagnosed with neonatal diabetes at birth or a few weeks after [1].

Neonatal diabetes mellitus (NDM)was first described by doctor Kitselle in1852, when his child presented with severe hyperglycemia at birth. From different studies scientists have concluded that thise form of diabetes results from mutations that occur on the surface of pancreatic beta cells. These can be KCNJ11, ABCC8, FOXP3 IBEX, GCK EIF2AK3 etc. [2].

Clinically two subgroups of NDM are recognised: transient and permanent. Transient NDM resolves at a median of 12 weeks but as many as 50%of cases will ultimately relapse. The majority ofpatients with transient NDM have an abnormality of imprintingof the ZAC and HYMAI genes on chromosome 6q.Permanent NDM requiresan ongoing insulin treatment when diagnosed. The commonest, but not the only one known cause of permanent NDMare mutations in the KCNJ11 gene encoding the Kir6.2 subunit of the β-cell KATP channel [1].

Due to the octamer structure of the pancreatic beta cell, the K ATP channels, which regulate insulin release, are affected by mutations in either the SUR1(ABCC8) or KCNJ11 (kir6.2) that will result in insufficient insulin release and anincreased calcium influx. In case of Wolcott-Rallison Syndrome the affected protein will be the eukaryotic translation initiation factor alpha kinase 3 (EIF2KA3),also known as PKR endoplasmic reticulum kinase. It is an autosomal recessive mutation which usually shows its first signs at the age of 3 months. 

Between January 2000 and August 2013 in the Institute of Biomedical and Clinical Science, University of Exeter Medical School, Exeter, UK genetic testing was conducted in 1020 patients (571 boys, 449 girls). This large, international, cohort studyincluded patients with neonatal diabetes diagnosed with diabetes before 6 months of age who were referred from 79 countries [3]. Mutations by comprehensive genetic testing, including Sanger sequencing, 6q24 methylation analysis, and targeted next-generation sequencing of all known neonatal diabetes genes we identified.Mutations in the potassium channel genes were the most common cause (n=390) of neonatal diabetes, but were identified less frequently in consanguineous families. In patients with genetically diagnosed Wolcott-Rallison syndrome, 23 (88%) of 26 patients tested within 3 months from the diagnosis had an isolated diabetes, compared with three (17%) of 18 patients referred later (>4 years; p<0·0001), in whom skeletal and liver involvement was common.

We present the fist case reported from Ukraine of a child diagnosed with permanent NDM resulting from a EIF2AK3 gene missense mutation of exon 15 (Wolcott-Rallison Syndrome).Confirmation of the gene mutation EIF2AK3 presence in our patient was done by the laboratoryof Exeter University, UK.

A diabetic boy was admitted for usual check up to the Endocrinology Department of Ternopil Regional Childrens’ Hospital.

From anamnesis: he is full-term child from second physiological pregnancy and delivery, birth weight 2,500 g. Neonatal period was usual. Family history is unremarkable. In the age of 6 weeks he was hospitalized due to moderate dehydration and intoxication syndromes without vomiting or diarrhea. Capillary blood glucose was 22 mmol/l (N=3,3-5,5) [396 mg/dl], Hb1Ac = 9.662 %.(N = 4,8-5,9),C-peptide = 0,27 ng/ml, (N = 0,9-7,10) and manifestation of NDM had been diagnosed. General condition of the child quickly improved due tothe combined insulin therapy. By genetic DNA analysis of parents and child blood novel EIF2AK3 gene missense mutation of exon 15 was revealed by Sanger sequencing which confirmed the clinical diagnosis of Wolcott-Rallison syndrome. The analysis of all other known neonatal diabetes genes did not identify a pathogenic mutation.

The child grew and developed properly on a regular basis-bolus insulin therapy in adaily dose of 0.36 U/kg body weight. The course of diabetes was stable, glycosylated hemoglobin per year observation was 8.2–8.5%.

In the age of 1 year the boy was hospitalized in the intensive care unit due to a grave general condition: fever, intoxication, lethargy, anorexia, jaundice, generalized edema, hepatomegaly, oliguria, acholic stool. A blood test revealed: mild hypochromic anemia, hypoproteinemia, normal urea and creatininelevel, high transaminases, hyperbilirubinemia, hypokaliemia. Hepatitis markers were negative. Glucose and ketone bodies were absent in the urine analyses. HbA1c was 8.6%.Hepatomegaly, portions of free liquid in abdominal and pericardial cavity, hydrocephaly were revealed at the ultrasound examination. Based on these signs and symptoms acute liver failure was diagnosed. 

After the intensive care for a month with insulin therapy, parenteral nutrition, repeated blood transfusions, albumin infusion, detoxification therapy, forced diuresis,correction of electrolyte balance,the child’s condition improved.

The mother complained of lameness of left leg in the child, which had become more intensive and persistent during the previous months. The x-ray examination confirmed the hypoplasia of the left hip joint.Bone dysplasia is one of the typical signs of Wolcott-Rallison Syndrome. The child was discharged from the hospital in a satisfactory condition.

In the age of two years, the child was hospitalized with an acute respiratory infection and sudden aggressive development of the liver failure which was fatal to the child despite of intensive care. 


Discussion

Most of all mutations at NDM are normally caused by intrauterine growth restriction, meaning these mutations usually occur during the first trimester. Early signs and symptoms in these infants include low weight, hyperglycemia, seizures, developmental delay and abnormal cognitive function. In the case of Wolcott-Rallison Syndrome, the children also do present with above stated signs but their distinctive features are osteopenia, bone dysplasia and, in most cases, a series of hepatic failures and splenomegaly. Although skeletal dysplasia is a main feature, it is usually noticed in patients in the period from 3 to 6years old. 

Wolcott-Rallison syndrome is an extremely rare condition worldwide. It was named after Drs Wolcott and Rallison, who first described this syndrome in three affected siblings [5]. It associates permanent neonatal or early-childhood insulin-dependent diabetes and epiphyseal dysplasia. Other clinical features that show variability among WRScases include mental retardation, hepatic and kidney dysfunction, cardiac abnormalities, exocrine pancreatic dysfunction, and neutropenia.Data on the epidemiology of WRS are limited, and the latest literature review on the subject suggested that less than 60 of WRS cases were reported worldwide [5–7]. However, the condition has been recently found to be the commonest genetic cause of PNDM in consanguineous families and in the Arab population [8]

In our case we couldnot’t find any confirmation of consanguineous marriage. Our patients were foundto be homozygous for a novel EIF2AK3 missense mutation p.G1010V has not been reported previously. But it is likely to a pathogenic since this glycine residue is highly conserved across species and a different mutation at this codon, p. G1010D has been identified by this laboratory in 2 patients with Wolcott-Rallison syndrome. 

There are only few case reports of the Wolcott-Rallison syndrome in which the clinical presentation includes recurrent infections, renal insuffiency, neutropenia, growth and developmental retardation, osteopenia, multiple epiphyseo-metaphyseal dysplasia, and exocrine pancreas insuffiency as common features [5,9]. Episodes of Acute Liver Failure are typical for this syndrome.     

Another specific features of Wolcott-Rallison syndrome is bone dysplasia [5].The multiple epiphyseal dysplasia associated withWRS characteristically affects the long bones, pelvis andvertebrae. Osteoporosis has also been described in association with WRS in some patients [10]

Genetic counselling, molecular analysis and sequencing, electrophysiological analysis and antenatal history can help in confirmation ofthe right diagnosis. Patients need managment with insulin and appropriate treatment for other associated conditions.Early genetic verification of the syndrome can be useful for individualized medical support of the patients and preventing early mortality by providing multiorgan transplantation, for example [11].


Conclusion

WRS should be suspected in any infant who presents with permanent neonatal diabetes associated with episodes of acute liver failure. Molecular genetic testing confirms the diagnosis. Early diagnostics is recommended in order to ensure rapid intervention for episodes of hepatic failure, which is the most life threatening complication.


We thank Drs. Kachor L.A., Sichkaruk L.V. and Globa Y.V. for providing clinical information and blood samples of the WRS patient. We thank the Exeter University Laboratory, UK, for genetic identification of diagnosis.

The study was presented: 55th ESPE annual meeting, 10-12 September, Paris, France 

Patient’s consent for publication has been granted

Contribution of authors must be listed on a separate form.

The authors contributed equally to this article. They read and approved the final version of the manuscript.

The authors declare that they have no competing interests

References

1. GlobalIDF/ISPAD guideline for diabetes in childhood and adolescence 2011 https://www.idf.org/sites/default/files/Diabetes-in-Childhood-and-Adolescence-Guidelines.pdf

2.  US Neonatal Diabetes Mellitus Registry http.//monogenicdiabetes.uchicago.edu/registry

3. De Franco E, Flanagan SE, Houghton JA et al. The effect of early, comprehensive genomic testing on clinical care in neonatal diabetes: an international cohort study. Lancet. 2015; 386(9997):957-963.

4. Dias RP, Buchanan CR, Thomas N et al. Os odontoideum in Wolcott-Rallison syndrome: a case series of 4 patients.Orphanet Journal of Rare Diseases. 2016;11:14.

5.  Wolcott CD, Rallison MV. Infancy-onset diabetes mellitus and multiple epiphyseal dysplasia. J Pediatr. 1972; 80:292-297.

6.  Julier C., Nicolino M.Wolcott-Rallison syndrome. Orphanet Journal of Rare Diseases 2010; 5:29http://www.ojrd.com/content/5/1/29

7. Brickwood S, Bonthron DT, Al-Gazali LI et al. Wolcott-Rallison syndrome: pathogenic insights into neonatal diabetes from new mutation and expression studies of EIF2AK3. J Med Genet. 2003;40:685-689.  

8.  Abdelhadi M. Habeb. Frequency and spectrum of Wolcott-Rallison syndrome in Saudi Arabia: a systematic review. Libyan Journal of Medicine. 2013; Volume8.ttp://www.libyanjournalofmedicine.net/index.php/ljm/article/view/21137.

9. Al-Sinani S, Al-Yaarubi S, Waadallah S et al. Novel Mutation in Wolcott-Rallison Syndrome with Variable Expression in Two Omani Siblings. Oman Medical Journal. 2015;30(2): 138-141.

10. Senée V, Vattem KM, Delépine M et al. Wolcott-Rallison Syndrome: clinical, genetic, and functional study of EIF2AK3 mutations and suggestion of genetic heterogeneity. Diabetes. 2004; 53(7):1876-1883.

11. Rivera E, Gupta S, Chavers B et al. En bloc multiorgan transplant (liver, pancreas, and kidney) for acute liver and renal failure in a patient with Wolcott-Rallison syndrome. Liver Transpl. 2016 Mar;22(3):371-374.

advanced search »

Article in databases

DOI: 10.18544/PEDM-22.03.0061
PUBMED: view

Similar articles

...

Growth hormone therapy in a girl with Turner syndrome and diabetes type ...

Transition of young adults with type 1 diabetes mellitus from a pediatri ...

Prevalence of chronic complications in patients with over 30-year histor ...

Yeast-like fungi in the gastrointestinal tract in children and adolescen ...

Pediatric Endocrinology Diabetes and Metabolism

2018; 24, 1: 1-51
2017; 23, 4: 169-222
2017; 23, 3: 117-168
2017; 23, 2: 59-116
2017; 23, 1: 1-58
2016; 22, 4: 133-180
2016; 22, 3: 81-132
2016; 22, 2: 43-79
2016; 22, 1: 1-42
2015; 21, 4: 149-191
2015; 21, 3: 97-148
2015; 21, 2: 51-96
2015; 21, 1: 1-50
2014; 20, 4: 131-182
2014; 20, 3: 83-130
2014; 20, 2: 35-82